Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + CDK6/CyclinD3 Kinase Enzyme System
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega BMX Kinase Enzyme System, 10microg
Recombinant full-length human BMX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells.
Promega ADP-Glo™ Kinase Assay + FLT3 (D835Y) Kinase Enzyme System
Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + ALK4 Kinase Enzyme System
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
Promega ADP-Glo™ Kinase Assay + HIPK3 Kinase Enzyme System
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega RET (V804L) Kinase Enzyme System, 10microg
Recombinant human RET (V804L) (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega CDK3/CyclinE1 Kinase Enzyme System, 10microg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega AMPK (A1/B1/G2) Kinase Enzyme System, 10microg
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
Promega TAK1-TAB1 Kinase Enzyme System, 10microg
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + TAOK1 Kinase Enzyme System
TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs).
Promega IKK beta Kinase Enzyme System, 10microg
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega HPK1 Kinase Enzyme System, 10microg
Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family.
Promega ADP-Glo™ Kinase Assay + CAMK1γ Kinase Enzyme System
Full-length recombinant human CAMK1 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK1 gamma (CLICK-III), a member of the CAMK family, is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase.
Promega ADP-Glo™ Kinase Assay + CAMK2α Kinase Enzyme System
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.
Promega ADP-Glo™ Kinase Assay + TGFβR1 Kinase Enzyme System
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.